Immune responses to the nonglycosylated ectodomain of respiratory syncytial virus attachment glycoprotein mediate pulmonary eosinophilia in inbred strains of mice with different MHC haplotypes
Identifieur interne : 003271 ( Main/Exploration ); précédent : 003270; suivant : 003272Immune responses to the nonglycosylated ectodomain of respiratory syncytial virus attachment glycoprotein mediate pulmonary eosinophilia in inbred strains of mice with different MHC haplotypes
Auteurs : Gerald E. Hancock [États-Unis] ; Paul W. Tebbey [États-Unis] ; Catherine A. Scheuer [États-Unis] ; Karin S. Pryharski [États-Unis] ; Kristen M. Heers [États-Unis] ; Natisha A. Lapierre [États-Unis]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2003-06.
Abstract
Development of subunit vaccines against respiratory syncytial virus (RSV) for naive human infants is hindered by concerns that immunization with the fusion or attachment (G) proteins will elicit polarized Type 2 T cell responses and cause immunopotentiation upon subsequent natural infection. We investigated the regions of G protein responsible for inducing a Type 2 T cell phenotype in inbred mice of different MHC haplotype toward development of vaccines with improved safety. As demonstrated by IL‐5‐dependent pulmonary eosinophilia after challenge and serum anti‐G protein IgG1 to IgG2 ratios, highly purified native G protein sensitized all strains for a Type 2 T cell phenotype. Stimulation of G protein‐primed splenocytes with synthetic overlapping peptides indicated that the nonglycosylated ectodomain was primarily responsible. Respectively the recall responses of BALB/c (H2d), C57BL/6 (H‐2b), SJL (H‐2s), and C3H/HeJ (H‐2k) mice were directed against epitopes within peptides spanning amino acids 184–198 (pep184–198), 168–181 (pep168–181) or 171–185 (pep171–185), 176–190 (pep176–190), and 104–118 (pep104–118) or 159–173 (pep159–173). Injection of pep184–198 conjugated to KLH (pep184–198‐KLH) primed H2d [BALB/c, B6.C‐H2d/bBy], but not H‐2b [C57Bl/6, C.B10‐H2b/LiMcd] mice for pulmonary eosinophilia. Sensitization with a peptide‐KLH conjugate encompassing amino acids 149–200 (pep149–200‐KLH) further confirmed that Type 2 T cell responses in BALB/c, C57BL/6 and SJL, but not C3H/HeJ mice were induced by the nonglycosylated ectodomain of G protein. These data are important for design of safe and efficacious subunit and attenuated vaccines for RSV. J. Med. Virol. 70: 301–308, 2003. © 2003 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.10395
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000467
- to stream Istex, to step Curation: 000467
- to stream Istex, to step Checkpoint: 000C76
- to stream Main, to step Merge: 003306
- to stream Main, to step Curation: 003271
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immune responses to the nonglycosylated ectodomain of respiratory syncytial virus attachment glycoprotein mediate pulmonary eosinophilia in inbred strains of mice with different MHC haplotypes</title><author><name sortKey="Hancock, Gerald E" sort="Hancock, Gerald E" uniqKey="Hancock G" first="Gerald E." last="Hancock">Gerald E. Hancock</name></author><author><name sortKey="Tebbey, Paul W" sort="Tebbey, Paul W" uniqKey="Tebbey P" first="Paul W." last="Tebbey">Paul W. Tebbey</name></author><author><name sortKey="Scheuer, Catherine A" sort="Scheuer, Catherine A" uniqKey="Scheuer C" first="Catherine A." last="Scheuer">Catherine A. Scheuer</name></author><author><name sortKey="Pryharski, Karin S" sort="Pryharski, Karin S" uniqKey="Pryharski K" first="Karin S." last="Pryharski">Karin S. Pryharski</name></author><author><name sortKey="Heers, Kristen M" sort="Heers, Kristen M" uniqKey="Heers K" first="Kristen M." last="Heers">Kristen M. Heers</name></author><author><name sortKey="Lapierre, Natisha A" sort="Lapierre, Natisha A" uniqKey="Lapierre N" first="Natisha A." last="Lapierre">Natisha A. Lapierre</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:5EFEB11FF42A2618D9C7F23189F1AEFE571DF348</idno><date when="2003" year="2003">2003</date><idno type="doi">10.1002/jmv.10395</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-RFF2BNMV-C/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000467</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000467</idno><idno type="wicri:Area/Istex/Curation">000467</idno><idno type="wicri:Area/Istex/Checkpoint">000C76</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C76</idno><idno type="wicri:doubleKey">0146-6615:2003:Hancock G:immune:responses:to</idno><idno type="wicri:Area/Main/Merge">003306</idno><idno type="wicri:Area/Main/Curation">003271</idno><idno type="wicri:Area/Main/Exploration">003271</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Immune responses to the nonglycosylated ectodomain of respiratory syncytial virus attachment glycoprotein mediate pulmonary eosinophilia in inbred strains of mice with different MHC haplotypes</title><author><name sortKey="Hancock, Gerald E" sort="Hancock, Gerald E" uniqKey="Hancock G" first="Gerald E." last="Hancock">Gerald E. Hancock</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Immunology Research, Wyeth Vaccines, West Henrietta</wicri:cityArea></affiliation><affiliation></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Correspondence address: Dept. of Immunology Research, Wyeth Research Vaccines, 211 Bailey Road, West Henrietta</wicri:cityArea></affiliation></author><author><name sortKey="Tebbey, Paul W" sort="Tebbey, Paul W" uniqKey="Tebbey P" first="Paul W." last="Tebbey">Paul W. Tebbey</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Immunology Research, Wyeth Vaccines, West Henrietta</wicri:cityArea></affiliation></author><author><name sortKey="Scheuer, Catherine A" sort="Scheuer, Catherine A" uniqKey="Scheuer C" first="Catherine A." last="Scheuer">Catherine A. Scheuer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Immunology Research, Wyeth Vaccines, West Henrietta</wicri:cityArea></affiliation></author><author><name sortKey="Pryharski, Karin S" sort="Pryharski, Karin S" uniqKey="Pryharski K" first="Karin S." last="Pryharski">Karin S. Pryharski</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Immunology Research, Wyeth Vaccines, West Henrietta</wicri:cityArea></affiliation></author><author><name sortKey="Heers, Kristen M" sort="Heers, Kristen M" uniqKey="Heers K" first="Kristen M." last="Heers">Kristen M. Heers</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Immunology Research, Wyeth Vaccines, West Henrietta</wicri:cityArea></affiliation></author><author><name sortKey="Lapierre, Natisha A" sort="Lapierre, Natisha A" uniqKey="Lapierre N" first="Natisha A." last="Lapierre">Natisha A. Lapierre</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Immunology Research, Wyeth Vaccines, West Henrietta</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">70</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="301">301</biblScope><biblScope unit="page" to="308">308</biblScope><biblScope unit="page-count">8</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="2003-06">2003-06</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Development of subunit vaccines against respiratory syncytial virus (RSV) for naive human infants is hindered by concerns that immunization with the fusion or attachment (G) proteins will elicit polarized Type 2 T cell responses and cause immunopotentiation upon subsequent natural infection. We investigated the regions of G protein responsible for inducing a Type 2 T cell phenotype in inbred mice of different MHC haplotype toward development of vaccines with improved safety. As demonstrated by IL‐5‐dependent pulmonary eosinophilia after challenge and serum anti‐G protein IgG1 to IgG2 ratios, highly purified native G protein sensitized all strains for a Type 2 T cell phenotype. Stimulation of G protein‐primed splenocytes with synthetic overlapping peptides indicated that the nonglycosylated ectodomain was primarily responsible. Respectively the recall responses of BALB/c (H2d), C57BL/6 (H‐2b), SJL (H‐2s), and C3H/HeJ (H‐2k) mice were directed against epitopes within peptides spanning amino acids 184–198 (pep184–198), 168–181 (pep168–181) or 171–185 (pep171–185), 176–190 (pep176–190), and 104–118 (pep104–118) or 159–173 (pep159–173). Injection of pep184–198 conjugated to KLH (pep184–198‐KLH) primed H2d [BALB/c, B6.C‐H2d/bBy], but not H‐2b [C57Bl/6, C.B10‐H2b/LiMcd] mice for pulmonary eosinophilia. Sensitization with a peptide‐KLH conjugate encompassing amino acids 149–200 (pep149–200‐KLH) further confirmed that Type 2 T cell responses in BALB/c, C57BL/6 and SJL, but not C3H/HeJ mice were induced by the nonglycosylated ectodomain of G protein. These data are important for design of safe and efficacious subunit and attenuated vaccines for RSV. J. Med. Virol. 70: 301–308, 2003. © 2003 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Hancock, Gerald E" sort="Hancock, Gerald E" uniqKey="Hancock G" first="Gerald E." last="Hancock">Gerald E. Hancock</name></region><name sortKey="Hancock, Gerald E" sort="Hancock, Gerald E" uniqKey="Hancock G" first="Gerald E." last="Hancock">Gerald E. Hancock</name><name sortKey="Heers, Kristen M" sort="Heers, Kristen M" uniqKey="Heers K" first="Kristen M." last="Heers">Kristen M. Heers</name><name sortKey="Lapierre, Natisha A" sort="Lapierre, Natisha A" uniqKey="Lapierre N" first="Natisha A." last="Lapierre">Natisha A. Lapierre</name><name sortKey="Pryharski, Karin S" sort="Pryharski, Karin S" uniqKey="Pryharski K" first="Karin S." last="Pryharski">Karin S. Pryharski</name><name sortKey="Scheuer, Catherine A" sort="Scheuer, Catherine A" uniqKey="Scheuer C" first="Catherine A." last="Scheuer">Catherine A. Scheuer</name><name sortKey="Tebbey, Paul W" sort="Tebbey, Paul W" uniqKey="Tebbey P" first="Paul W." last="Tebbey">Paul W. Tebbey</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003271 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003271 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:5EFEB11FF42A2618D9C7F23189F1AEFE571DF348
|texte= Immune responses to the nonglycosylated ectodomain of respiratory syncytial virus attachment glycoprotein mediate pulmonary eosinophilia in inbred strains of mice with different MHC haplotypes
}}
| This area was generated with Dilib version V0.6.33. |  |